Awaiting product image
, , , , ,

AmpliMax FFPE DNA Extraction Kit

AmpliMaxAutomated · Magnetic bead

Automated magnetic-bead DNA extraction from FFPE tissue sections and curls, with deep-well plates and tip combs included.

Pack size Catalogue number
64 preparations AM-FFPD-64
SKU: AM-FFPD-64 Categories: , , , , ,

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa

Overview

Formalin-fixed, paraffin-embedded tissue is a demanding source of nucleic acid because fixation, paraffin embedding and prolonged storage can reduce nucleic-acid accessibility. The AmpliMax FFPE DNA Extraction Kit pairs a defined FFPE sample preparation with automated magnetic-bead purification for FFPE tissue sections and curls.

Deparaffinisation, Proteinase K digestion and heat treatment prepare the sample on the bench. The clarified lysate is then loaded onto the extraction system, which carries out binding, washing and elution, reducing repetitive manual handling for research laboratories processing FFPE material.

Key benefits

Amplifiable DNA from archived tissue
A dedicated 90 °C crosslink reversal step frees DNA from formalin damage before capture, so more of each FFPE sample can be used for PCR and NGS.
Runs on open extraction systems
Runs on the Tianlong Libex, Seegene SEEPrep32 and Aurora Biomed ADNap 20, rather than a single dedicated instrument.
Local manufacturer, direct support
Manufactured in South Africa by Gene Vantage. Speak directly to the scientists who formulate the reagents, and ask about custom pack sizes or instrument setups.
RNA-free DNA when needed
Add user-supplied RNase A after the heat step to remove co-purified RNA.

How it works

FFPE material is deparaffinised and rehydrated before enzymatic digestion and high-temperature crosslink reversal. The clarified lysate is combined with binding buffer and magnetic beads and loaded onto the automated system, which completes binding, washing and elution.

Workflow

1Deparaffinise2Rehydrate3Lyse and digest4Reverse formalin crosslinks5Clarify lysate6Automated bead purification7Elute

Other automated systems

Running this kit on another open automated system? Please contact Gene Vantage before the first run to discuss programming.

Measuring FFPE DNA

FFPE DNA is fragmented and partly damaged, so UV-vis and fluorometric readings do not reliably show how much DNA will amplify. Where possible, check FFPE DNA by qPCR before downstream use. Fragmented DNA suits short-amplicon PCR and NGS, not long-amplicon assays.

Contents and storage

Component Per sample Storage
Lysis KPH 200 µL 15-25 °C
Proteinase K 20 µL 4-8 °C
Bind C 200 µL 15-25 °C
Booster L 20 µL 4-8 °C
Magnetic Beads 30 µL 4-8 °C
Wash NovaYM 600 µL 15-25 °C
Wash Ultimus 600 µL 15-25 °C
Elution Buffer 70 µL 15-25 °C
2.2 mL deep-well plate 2 per kit 15-25 °C
8-strip tip comb 8 per kit 15-25 °C

All reagents ship at ambient temperature.

At a glance

Sample typeFFPE tissue sections and curls
Nucleic acidDNA
FormatMagnetic bead
Platform compatibilityTianlong Libex; Seegene SEEPrep32; Aurora Biomed ADNap 20; other open automated systems, including KingFisher, Hamilton and Tecan
Elution volume70 µL
GradeResearch (RUO)

Full details in the Specifications tab.

Ordering

64 preparationsAM-FFPD-64

To order or request a test kit: sales@gene-vantage.com

You supply

Reagents

Xylene or other deparaffinisation reagent
Ethanol
Isopropanol
RNase A, 20 mg/mL (optional)

Equipment

Tianlong Libex, Seegene SEEPrep32, Aurora Biomed ADNap 20 or another open automated system
Microcentrifuge capable of 12,800 × g (11,000 rpm)
Thermomixer or heating block capable of 56 °C and 90 °C
Vortex mixer
Micropipettes (10 µL to 1,000 µL)
Fume hood or well-ventilated enclosure for xylene handling

Consumables

1.5 mL microcentrifuge tubes
Aerosol-barrier filter tips (10 µL to 1,000 µL)
Automation-compatible deep-well plates

Applications

PCRqPCRDNA sequencingNGS library preparation

Sample type

FFPE tissue sections and curls
Sample input

4 to 6 sections
Nucleic acid

DNA
Format

Magnetic bead
Workflow

Automated
Platform compatibility

Tianlong Libex; Seegene SEEPrep32; Aurora Biomed ADNap 20; other open automated systems, including KingFisher, Hamilton and Tecan
Elution volume

70 µL
Pack size

64 preparations
Grade

Research (RUO)
Intended use

For Research Use Only. Not for use in diagnostic procedures.
Shelf life and storage

12 months. Storage: check individual components (see Contents and storage)
Applications

PCR, qPCR, DNA sequencing, NGS library preparation

Related products

Shopping Basket
Scroll to Top