Overview
The band you cut from a gel is the material your next experiment depends on, and any agarose or salt that comes with it can stop the ligation, sequencing or PCR that follows.
The IsoBind Gel Extraction Kit recovers your fragment free of the gel, so the next reaction gets clean template.
Key benefits
Keep only the fragment you need
Recovers the single band you cut from the gel, so only your chosen fragment goes into the next step.
Uses equipment you already have
Uses a microcentrifuge, heating block and other standard laboratory equipment, with no dedicated instrument to buy.
Any band you cut
PCR products, restriction fragments and other bands all go through the same protocol.
Concentrated eluate
Elutes in 50 µL, so the recovered fragment stays concentrated for ligation, sequencing or PCR.
How it works
Excise the chosen band and dissolve the agarose. Add binding buffer, load the spin column, then wash, dry and elute the purified fragment.
Workflow
1Excise the selected band2Dissolve the agarose3Prepare the sample for binding4Load the spin column5Wash the membrane6Dry the membrane7Elute the fragment
Before first use
Add ethanol to the wash buffer concentrate and isopropanol to the binding buffer, as described in the manual. Mark each bottle once prepared.
Protect the fragment in your gel
Trim away unnecessary agarose and use blue-light illumination where possible. If using UV light, keep exposure brief to limit damage to the fragment.
Contents and storage
| Component |
Per sample |
Storage |
| Gel Melt Buffer |
820 µL |
15-25 °C |
| Bind C |
560 µL |
15-25 °C |
| Wash Ultimus |
700 µL |
15-25 °C |
| Elution Buffer |
50 µL |
15-25 °C |
| Spin column |
1 |
15-25 °C |
All reagents ship at ambient temperature.
Product at a glance
Sample typeAgarose gel slices
Nucleic acidDNA and RNA fragments, including PCR products
FormatSilica spin column
WorkflowManual spin-column workflow
Elution volume50 µL
GradeResearch (RUO)
Full details in the Specifications tab.
Why laboratories choose Gene Vantage
Technical answers from our scientists
Expert troubleshooting directly from the scientists behind your reagents.
Locally manufactured. Locally supplied.
Less reliance on overseas shipments and import lead times.
High-throughput and specialist applications
Tailored formats for larger-scale and specialist projects, where practical.
Quality built in
From formulation to packaging, we build performance and value into every kit.
Ordering
50 preparationsIB-GEL-50
100 preparationsIB-GEL-100
To order or request a test kit: sales@gene-vantage.com
You supply
Reagents
Ethanol
Isopropanol
Equipment
Blue-light or UV transilluminator
Clean scalpel or blade
Balance accurate to 1 mg
Heating block or water bath at 55 °C
Heating block at 70 to 90 °C
Microcentrifuge capable of 12,800 × g (11,000 rpm)
Vortex mixer
Calibrated pipettes
Consumables
1.5 mL tubes, two per sample (one nuclease-free)
Aerosol-barrier filter tips
Applications
Recovery of PCR products and restriction fragments from agarose gels
Fragments for cloning and ligation
Templates for sequencing and PCR
