Overview
Human DNA samples now arrive as saliva, dried blood spot cards and small blood volumes, often from collection points far from the laboratory.
The AmpliMax KingPlex Human DNA Kit handles all three on KingFisher Flex and KingFisher Apex, so samples collected without needles or posted on cards are processed alongside whole blood.
Key benefits
Three specimen types, one kit
Saliva, dried blood spot cards and small-volume whole blood run through one kit, so one extraction protocol serves all three.
Runs on your KingFisher
Runs on KingFisher Flex and KingFisher Apex, so human DNA extraction fits the instruments you already use.
DNA for genotyping and pharmacogenetics
Genomic DNA suits PCR-based human genotyping and pharmacogenetic research.
No needles needed for saliva
Saliva and dried blood spot cards can be collected without venepuncture, so collection is simple for donors and staff.
How it works
Saliva, dried blood spot material or small-volume whole blood is prepared and lysed before magnetic-bead purification. DNA is captured, washed and eluted on the extraction system.
Workflow
1Lyse the sample2Add magnetic beads3Load the extraction system4Bind to magnetic beads5Wash6Elute
Before first use
Add ethanol to the wash buffer concentrates, as described in the manual. Mark each bottle once prepared.
Using another instrument?
This KingPlex kit runs on KingFisher Flex and KingFisher Apex. For other open automated systems, ask Gene Vantage about an AmpliMax kit for your instrument.
Contents and storage
| Component |
Per sample |
Storage |
| Proteinase K |
30 µL |
4-8 °C |
| Booster L |
10 µL |
4-8 °C |
| Wash Ultimus |
600 µL |
15-25 °C |
| Elution Buffer |
100 µL |
15-25 °C |
| Lysis KPH |
425 µL |
15-25 °C |
| Magnetic Beads |
40 µL |
4-8 °C |
| Wash NovaYM |
1000 µL |
15-25 °C |
All reagents ship at ambient temperature.
Product at a glance
Sample typeSaliva, dried blood spot cards and small-volume whole blood
Nucleic acidDNA
FormatMagnetic bead
Platform compatibilityKingFisher Flex; KingFisher Apex
Elution volume70 µL
GradeResearch (RUO)
Full details in the Specifications tab.
Why laboratories choose Gene Vantage
Technical answers from our scientists
Expert troubleshooting directly from the scientists behind your reagents.
Locally manufactured. Locally supplied.
Less reliance on overseas shipments and import lead times.
High-throughput and specialist applications
Tailored formats for larger-scale and specialist projects, where practical.
Quality built in
From formulation to packaging, we build performance and value into every kit.
Ordering
100 preparationsAM-HDNA-KP-100
384 preparationsAM-HDNA-KP-384
To order or request a test kit: sales@gene-vantage.com
You supply
Reagents
✓Ethanol
Equipment
✓Clean paper punch or sterile scissors
✓Heating block or water bath at 60 °C
✓Vortex mixer
✓KingFisher Flex or KingFisher Apex magnetic particle processor
✓Calibrated pipettes
✓Magnetic rack for 1.5 mL tubes (optional)
Consumables
✓Sterile saliva collection tubes or vials
✓1.5 mL tubes, two per sample
✓KingFisher plates and tip comb, new for each run
✓Aerosol-barrier filter tips
Applications
✓Human genotyping and pharmacogenetic research by PCR
✓DNA extraction from saliva, dried blood spots and whole blood
✓Population and cohort studies
