Overview
HPV testing depends on DNA from cervical swabs and Pap smear material, and many laboratories that offer it do not have an automated extractor.
The IsoBind HPV DNA Kit is built for these specimens, so laboratories without an extractor can offer HPV PCR and genotyping.
Key benefits
Made for HPV testing
Formulated for cervical swabs, Pap smear material and epithelial cells, the specimens HPV testing starts from.
Uses equipment you already have
Runs on a standard microcentrifuge, with no extraction instrument to buy.
For diagnostic laboratories
IVD grade, made for diagnostic laboratories running HPV detection and genotyping.
Concentrated DNA
Elutes in 50 µL, so the extract stays concentrated for HPV PCR.
How it works
Cervical or epithelial specimens are prepared and lysed with Proteinase K. The DNA-containing sample is prepared for silica binding, transferred to the spin column, washed, dried and eluted.
Workflow
1Cervical specimen preparation2Binding preparation3Spin-column loading4Sequential washes5Membrane drying6DNA elution
Before first use
Add ethanol to the wash buffer concentrates and isopropanol to the binding buffer, as described in the manual. Mark each bottle once prepared.
Collection media
Check that your collection and transport medium suits this kit before you start testing, or ask Gene Vantage.
Contents and storage
| Component |
Per sample |
Storage |
| Bind C |
300 µL |
15-25 °C |
| Booster L |
20 µL |
4-8 °C |
| Elution Buffer |
160 µL |
15-25 °C |
| OrionSD12 |
200 µL |
15-25 °C |
| Proteinase K |
20 µL |
4-8 °C |
| Wash D |
500 µL |
15-25 °C |
| Wash NovaY |
500 µL |
15-25 °C |
| Wash Ultimus |
500 µL |
15-25 °C |
| Spin column |
1 |
15-25 °C |
All reagents ship at ambient temperature.
Product at a glance
Sample typeCervical swabs, Pap smear samples, epithelial cell suspensions
Nucleic acidDNA
FormatSilica spin column
WorkflowManual
Elution volume50 µL
GradeIVD
Full details in the Specifications tab.
Why laboratories choose Gene Vantage
Technical answers from our scientists
Expert troubleshooting directly from the scientists behind your reagents.
Locally manufactured. Locally supplied.
Less reliance on overseas shipments and import lead times.
High-throughput and specialist applications
Tailored formats for larger-scale and specialist projects, where practical.
Quality built in
From formulation to packaging, we build performance and value into every kit.
Ordering
100 preparationsIB-HPV-100
200 preparationsIB-HPV-200
250 preparationsIB-HPV-250
To order or request a test kit: sales@gene-vantage.com
You supply
Reagents
✓Ethanol
✓Isopropanol
Equipment
✓Microcentrifuge capable of 12,800 × g (11,000 rpm)
✓Vortex mixer
✓Heating block, thermomixer, oven, or water bath for 56 °C incubation
✓Pipettes
Consumables
✓1.5 mL RNase-free microcentrifuge tubes
✓Aerosol-barrier pipette tips
Applications
✓HPV detection and genotyping by PCR
✓DNA extraction from cervical swabs and Pap smear material
