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IsoBind FFPE DNA Extraction Kit

IsoBindManual · Silica spin column

Manual silica spin-column DNA purification from FFPE tissue sections and curls, with deparaffinisation, digestion and crosslink reversal.

Pack size Catalogue number
50 preparations IB-FFPD-50
250 preparations IB-FFPD-250

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa
Request a quote

Or email sales@gene-vantage.com with catalogue number IB-FFPD.

Overview

Formalin-fixed, paraffin-embedded tissue is a demanding source of DNA because fixation, paraffin embedding and prolonged storage can reduce nucleic-acid accessibility. The IsoBind FFPE DNA Extraction Kit provides a dedicated manual workflow for FFPE tissue sections and curls.

Deparaffinisation, Proteinase K digestion and heat treatment prepare the sample before silica spin-column purification, giving research laboratories a route from FFPE material to purified DNA using standard benchtop equipment.

Key benefits

Established FFPE chemistry
Proteinase K digestion at 56 °C, then crosslink reversal at 90 °C for 1 hour: the widely used conditions for recovering amplifiable DNA from FFPE tissue.
No instrument to buy
Runs on standard benchtop equipment, with no extraction instrument to buy.
DNA for sequencing
Purified DNA is suitable for PCR, qPCR, DNA sequencing and NGS library preparation.
RNA-free DNA when needed
Add user-supplied RNase A at the lysis step to remove co-purified RNA.

How it works

Deparaffinise with xylene or your laboratory's own deparaffinisation reagent. FFPE material is then rehydrated before enzymatic digestion and high-temperature crosslink reversal. The clarified lysate is combined with binding buffer and loaded onto the silica spin column, which is then washed, dried and eluted.

Technical details for approved customers

Log in or request access to see kit contents, per-sample volumes, storage, what you need to supply, workflow steps and handling notes.

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Product at a glance

Sample typeFFPE tissue sections and curls
Nucleic acidDNA
FormatSilica spin column
WorkflowManual
Elution volume50 µL
GradeResearch (RUO)

Full details in the Specifications tab.

Why laboratories choose Gene Vantage

Technical answers from our scientists
Expert troubleshooting directly from the scientists behind your reagents.
Locally manufactured. Locally supplied.
Less reliance on overseas shipments and import lead times.
High-throughput and specialist applications
Tailored formats for larger-scale and specialist projects, where practical.
Quality built in
From formulation to packaging, we build performance and value into every kit.

Ordering

50 preparationsIB-FFPD-50
250 preparationsIB-FFPD-250

To order or request a test kit: sales@gene-vantage.com

Applications

PCR
qPCR
DNA sequencing
NGS library preparation
Sample type

FFPE tissue sections and curls

Sample input

4 to 6 FFPE sections

Nucleic acid

DNA

Format

Silica spin column

Workflow

Manual

Elution volume

50 µL

Pack size

50 or 250 preparations

Grade

Research (RUO)

Intended use

For Research Use Only. Not for use in diagnostic procedures.

Shelf life and storage

12 months. Storage: check individual components (see Contents and storage)

Applications

PCR, qPCR, DNA sequencing, NGS library preparation

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