Proteinase K Solution

ReagentsReagent only

Ready-to-use Proteinase K solution for digesting proteins and inactivating nucleases during DNA and RNA extraction.

Pack size	Catalogue number
1 mL	RE-PROK-1
5 mL	RE-PROK-5

Specialists in the field of nucleic acid extractions

only manufacturer of such products in Africa
1-3 Day Delivery times in South Africa

Request a quote

Or email sales@gene-vantage.com with catalogue number RE-PROK.

Overview
Specifications

Overview

Proteins and nucleases released when a sample is lysed can hold back and degrade DNA and RNA before they reach purification. Proteinase K is a broad-spectrum serine protease that digests these proteins, so the nucleic acids are released intact.

Gene Vantage Proteinase K is supplied as a ready-to-use solution, so there is no powder to weigh or dissolve, and it can be added to your own genomic DNA and RNA extraction methods.

Key benefits

Active in strong lysis buffers

Stays active in the SDS, urea and chaotropic salt buffers used in spin column and magnetic bead extraction.

Not stopped by EDTA

EDTA does not inactivate the enzyme, so it works in lysis buffers that contain EDTA.

Flexible dosing

Use 20 or 30 µL per sample as Gene Vantage kits do, or the amount your own method specifies.

Same enzyme as our kits

The Proteinase K supplied in Gene Vantage extraction kits, available on its own for your other methods.

How it works

Add Proteinase K to the sample in lysis buffer, mix and incubate at 56 to 60 °C until the sample is fully lysed, then continue with binding, washing and elution. The wash steps remove the enzyme.

Technical details for approved customers

Product at a glance

Storage4 to 8 °C

Shelf life12 months

GradeResearch (RUO)

Full details in the Specifications tab.

Why laboratories choose Gene Vantage

Technical answers from our scientists

Expert troubleshooting directly from the scientists behind your reagents.

Locally manufactured. Locally supplied.

Less reliance on overseas shipments and import lead times.

High-throughput and specialist applications

Tailored formats for larger-scale and specialist projects, where practical.

Quality built in

From formulation to packaging, we build performance and value into every product.

Ordering

1 mLRE-PROK-1

5 mLRE-PROK-5

To order or request a test kit: sales@gene-vantage.com

Applications

Genomic DNA extraction

RNA extraction

Protein and nuclease removal

Format	Reagent only
Pack size	1 mL or 5 mL
Grade	Research (RUO)
Intended use	For Research Use Only. Not for use in diagnostic procedures.
Shelf life and storage	12 months from manufacture in the unopened container, stored at 4 to 8 °C. After opening: close tightly, store at 4 to 8 °C, never return unused liquid, and use before the expiry date.
Applications	Genomic DNA extraction, RNA extraction, Protein and nuclease removal