Overview
When PCR products are cleaned up one tube at a time, clean-up ties up a person who could be doing something else.
The AmpliMax Post PCR Cleanup Kit moves that step onto the extraction system you already own, so whole batches are cleaned up together and passed straight on to sequencing or further PCR.
Key benefits
Walk-away clean-up
Once samples are loaded, the extraction system does the work, so staff are free while the batch runs.
Runs on open extraction systems
Runs on the Tianlong Libex, Seegene SEEPrep32 and Aurora Biomed ADNap 20, rather than a single dedicated instrument.
No columns to handle
Magnetic beads replace spin columns, so there is no column to move for each sample.
Ready for sequencing
Purified PCR products are ready for sequencing and other downstream steps.
How it works
The PCR product is combined with binding buffer and magnetic beads and loaded onto the automated system, which completes binding, washing and elution.
Workflow
1Prepare the PCR product2Add binding buffer and beads3Load the extraction system4Automated bead purification5Elute
Before first use
Add ethanol to the wash buffer concentrates before the first run. Contact Gene Vantage for the volumes for your pack size.
Other automated systems
Running this kit on another open automated system? Please contact Gene Vantage before the first run to discuss programming.
Contents and storage
| Component |
Per sample |
Storage |
| Post PCR Cleanup Buffer |
500 µL |
15-25 °C |
| Magnetic Beads |
20 µL |
4-8 °C |
| Wash D |
500 µL |
15-25 °C |
| Wash Ultimus |
500 µL |
15-25 °C |
| Elution Buffer |
50 µL |
15-25 °C |
All reagents ship at ambient temperature.
Product at a glance
Sample typePCR product
Nucleic acidDNA
FormatMagnetic bead
Platform compatibilityTianlong Libex; Seegene SEEPrep32; Aurora Biomed ADNap 20; other open automated systems, including KingFisher, Hamilton and Tecan
Elution volume50 µL
GradeResearch (RUO)
Full details in the Specifications tab.
Why laboratories choose Gene Vantage
Technical answers from our scientists
Expert troubleshooting directly from the scientists behind your reagents.
Locally manufactured. Locally supplied.
Less reliance on overseas shipments and import lead times.
High-throughput and specialist applications
Tailored formats for larger-scale and specialist projects, where practical.
Quality built in
From formulation to packaging, we build performance and value into every kit.
Ordering
100 preparationsAM-PPC-100
500 preparationsAM-PPC-500
To order or request a test kit: sales@gene-vantage.com
You supply
Reagents
✓Ethanol
Equipment
✓Automated magnetic bead extraction system: Tianlong Libex, Seegene SEEPrep32, Aurora Biomed ADNap 20, or another open automated system, including KingFisher, Hamilton and Tecan (contact Gene Vantage before the first run)
✓Vortex mixer
✓Calibrated pipettes
✓Magnetic rack for 1.5 mL tubes (optional)
Consumables
✓1.5 mL tubes, two per sample
✓Deep-well plate (96-well) and tip comb of the type the instrument requires, new for each run
✓Aerosol-barrier filter tips
Applications
✓Batch clean-up of PCR products on automated extraction systems
✓Clean-up of PCR products before sequencing
