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AmpliMax Large Seed DNA Kit 100-Prep

Generate PCR-ready genomic DNA from maize, sunflower and other large-kernel seed tissue with a magnetic bead workflow that scales from a manual stand to open automation. Mechanical disruption and Booster-assisted capture help manage the oils, starch and polysaccharides that make large seeds difficult to process. The purified DNA is ready for variety registration, pedigree tracking, seed quality screening and high-throughput genotyping.

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa

Built for breeding and seed-testing laboratories, the AmpliMax Large Seed DNA Kit provides a scalable magnetic bead route from mechanically disrupted large seed tissue to analysis-ready genomic DNA.

Samples are homogenised before a 65°C lysis step. Booster Compound supports magnetic bead binding in inhibitor-rich lysates, followed by two wash steps that reduce oils, starch and polysaccharide carryover. The same chemistry can be run on a magnetic stand or transferred to a compatible open automation platform as throughput grows.

The kit is intended for maize, sunflower and other large-kernel crop samples, including high-oil and high-starch seeds. Processing time is platform-dependent, from approximately 20 minutes for the magnetic bead stage to larger automated workflows.

Recovered DNA is suitable for variety registration, pedigree tracking, seed quality screening and genotyping arrays. Local manufacture supports dependable supply for agricultural programmes.

Sample type

Agricultural seed

Nucleic acid

DNA

Format

Magnetic bead

Workflow

Manual or Automated

Compatibility

Universal

Pack size

100 samples

Grade

Agricultural

Applications

Research, Agriculture, Food & Beverage, Forensics

Required, not supplied

Items you provide to complete the workflow.

  • Isopropanol
  • Absolute ethanol
  • Filter tips
  • 2 ml Safelock tubes
  • Reducing agent (DTT, BME optional)
  • Robotic system (automated)
  • Magnetic stand (manual)
  • Pipettes
  • Microcentrifuge ≥10,000×g
  • Sample crushing device
  • Vortex
  • Oven or heating block
  • Steel beads for crushing
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