Overview
Viral load and blood-borne virus testing start from small amounts of viral RNA in serum or plasma, which must come through extraction intact.
The IsoBind Viral RNA Kit is built for these cell-free blood specimens, so viral RNA reaches your RT-qPCR assay intact.
Key benefits
Made for serum and plasma
Formulated for human serum and plasma, the specimens blood-borne RNA virus testing starts from.
Uses equipment you already have
Runs on a standard microcentrifuge, with no extraction instrument to buy.
RNA for viral load testing
Purified RNA is suitable for RT-qPCR viral load testing and RT-PCR detection of blood-borne RNA viruses.
For diagnostic laboratories
IVD grade, made for diagnostic laboratories testing serum and plasma for RNA viruses.
How it works
The specimen is lysed, then combined with a proprietary binding solution and applied to a silica spin column. The membrane is washed, dried and eluted to recover viral RNA.
Workflow
1Prepare working solutions2Add specimen and lyse3Bind RNA to the silica membrane4Wash the column membrane5Dry the membrane6Elute viral RNA
Before first use
Add alcohol to the binding solution and the wash buffer concentrates, as described in the manual. Mark each bottle once prepared. DNase is not included: if your assay needs DNA-free RNA, add a DNase step as described in the manual.
Serum and plasma
Use the collection and handling conditions given in the manual. Contact Gene Vantage before using another specimen type.
Contents and storage
| Component |
Per sample |
Storage |
| Lysis NovaB05 |
200 µL |
15-25 °C |
| Bind C |
300 µL |
15-25 °C |
| Wash NovaY |
400 µL |
15-25 °C |
| Wash Ultimus |
400 µL |
15-25 °C |
| Elution Buffer |
100 µL |
15-25 °C |
| Proteinase K |
20 µL |
4-8 °C |
| Booster L |
10 µL |
4-8 °C |
| Wash D |
400 µL |
15-25 °C |
| Spin column |
1 |
15-25 °C |
All reagents ship at ambient temperature.
Product at a glance
Sample typeHuman serum and plasma
Nucleic acidRNA
FormatSilica spin column
WorkflowManual spin-column workflow
Elution volume50 µL
GradeIVD
Full details in the Specifications tab.
Why laboratories choose Gene Vantage
Technical answers from our scientists
Expert troubleshooting directly from the scientists behind your reagents.
Locally manufactured. Locally supplied.
Less reliance on overseas shipments and import lead times.
High-throughput and specialist applications
Tailored formats for larger-scale and specialist projects, where practical.
Quality built in
From formulation to packaging, we build performance and value into every kit.
Ordering
50 preparationsIB-VRNA-50
100 preparationsIB-VRNA-100
To order or request a test kit: sales@gene-vantage.com
You supply
Reagents
✓Ethanol
✓Alcohol for the binding solution, as described in the manual
✓DNase
Equipment
✓Microcentrifuge capable of 12,800 × g (11,000 rpm)
✓Vortex mixer
✓Thermomixer, heating block, oven, or water bath for controlled-temperature incubation
✓Pipettes
Consumables
✓1.5 mL RNase-free microcentrifuge tubes
✓Aerosol-barrier (RNase-free) pipette tips
Applications
✓Viral load testing by RT-qPCR
✓Detection of blood-borne RNA viruses by RT-PCR
✓Viral RNA extraction from serum and plasma
