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AmpliMax KingPlex Human DNA Kit (IVD) 384-Prep

Prepare genomic DNA from saliva, dried blood spot cards and small-volume whole blood samples.

  • Automated magnetic bead workflow for low volume input samples
  • Validated on Tianlong Libex and Seegene SEEPrep32
  • Compatible with KingFisher, Opentrons, Bioer, Zhengkebio and other open platforms (Hamilton, Tecan etc).
  • Sample input includes 120 ul raw saliva, 100 µL whole blood, 2 x 4mm discs DBS/Whatman cards
  • Suitable for genotyping, STR analysis, NGS sequencing and other downstream DNA applications

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa

The AmpliMax KingPlex Human DNA Kit prepares genomic DNA from saliva, dried blood spot cards and small-volume whole blood samples using magnetic bead chemistry. The workflow combines extended lysis, magnetic capture and controlled washing to prepare DNA for downstream analysis. A proprietary nucleic acid recovery enhancer promotes DNA capture, particularly when sample input is limited.

The method has been validated on Tianlong Libex and Seegene SEEPrep32. It is also compatible with KingFisher, Revvity chemagic 360, Opentrons, Bioer, Zhengkebio, BioPerfectus, AdNAP, Snibe, chemagic360 and configurable liquid handlers from Hamilton, Beckman Coulter, Tecan, LGC and Opentrons. For configurable liquid handlers, the user laboratory is responsible for instrument programming, labware selection and local workflow verification.

Compatible with Whatman™ 903 Protein Saver Card (05-715-121), Whatman™ 903 Neonatal Screening Cards (09-800-961) and Whatman™ FTA Classic Cards (09-923-341 or 09-923-340).

Purified DNA is suitable for genotyping, STR analysis and other downstream DNA applications. Local manufacture supports shorter lead times, reliable supply and direct access to technical support.

Application

Isolation of genomic DNA

Target

DNA

Sample material

Saliva, dried blood spot cards and small-volume whole blood

Sample amount

120 ul raw saliva, 100 µL whole blood, 2 x 4mm discs DBS/Whatman cards

Automated use

Yes

Automation compatibility

Compatible with KingFisher, KingFisher, Chemagic 360, Bioer, Zhengkebio, BioPerfectus, AdNAP, Snibe and configurable liquid handlers from Hamilton, Beckman Coulter, Tecan, LGC and Opentrons. End-user laboratory programming and local verification are required on configurable liquid handlers.

Validated systems

Tianlong Libex and Seegene SEEPrep32

Elution volume

40 – 100 µL (adjustable for concentration or overall recovery)

Format

Magnetic separation

Fragment size

300 bp to ± 30 kbp

Handling

Automated magnetic capture, with manual sample pre-treatment as required

Technology

Magnetic bead chemistry

Binding potential

0.4 µg/µL beads

Typical downstream applications

Genotyping, STR analysis, multiplex PCR, Southern blotting, enzymatic reactions & NGS

Shipping temperature

15–25 °C / 59–77 °F

Required, not supplied

Items you provide to complete the workflow.

  • Isopropanol
  • Absolute ethanol
  • Filter tips
  • Deepwell plates for Kingfisher system
  • Reducing agent (DTT, BME optional)
  • Thermo Kingfisher system
  • Pipettes
  • Centrifuge
  • Vortex
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