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IsoBind FFPE DNA Kit (IVD) 100-Prep

Recover amplifiable DNA from 5-10 µm FFPE tissue sections with a manual silica spin-column workflow that avoids xylene. Deparaffinisation and heat-based de-crosslinking address paraffin and formalin crosslinks before the DNA is captured and washed. The approximately 60-minute workflow prepares DNA for oncology mutation profiling, somatic variant analysis and targeted sequencing.

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa

The IsoBind FFPE DNA Kit is designed for pathology and molecular laboratories that need usable genomic DNA from archival formalin-fixed, paraffin-embedded tissue. It combines xylene-free sample preparation with manual silica spin-column purification.

FFPE sections first undergo proprietary deparaffinisation, Proteinase K digestion and a 90°C de-crosslinking step. These stages remove paraffin and partially reverse formalin-induced crosslinks before the DNA is bound to the silica membrane, washed and eluted.

The kit processes 5-10 µm FFPE sections, with core biopsies and cytology blocks as secondary inputs. The complete workflow takes approximately 60 minutes on a standard laboratory centrifuge and is supplied for in vitro diagnostic use.

Purified DNA is suitable for oncology mutation profiling, somatic variant detection, pathogen identification from archival specimens and targeted sequencing. Manufactured to international standards, with fast local access to technical support.

Sample type

FFPE

Nucleic acid

DNA

Format

Silica spin column

Workflow

Manual

Compatibility

Universal

Pack size

100 samples

Grade

Medical (IVD)

Applications

Diagnostics, Next generation sequencing, Healthcare

Required, not supplied

Items you provide to complete the workflow.

  • Isopropanol
  • Absolute ethanol
  • Filter tips
  • Microtubes
  • Pipettes
  • Microcentrifuge ≥10,000×g
  • Microcentrifuge (spin columns)
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