next generation sequencing
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IsoBind Gel Extraction Kit 50-Prep
Recover DNA from 0.5-2% agarose gel slices in approximately 20 minutes with a manual silica spin-column clean-up workflow. Gel Melt Buffer dissolves the agarose before DNA binding, while the wash step removes dye, salts and residual gel contaminants. The purified DNA is ready for cloning, sequencing, PCR and library preparation.
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IsoBind PCR Purification Kit 50-Prep
Remove primers, dNTPs, polymerase and salts from PCR reactions in approximately 15 minutes with a manual silica spin-column clean-up workflow. The four-step bind, wash and elute process handles DNA fragments from 150 bp to 5 kb and concentrates them in a 40 µL elution. Purified DNA is ready for Sanger sequencing, cloning, enzymatic reactions and gel-free fragment analysis.
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IsoBind PCR Purification Kit 100-Prep
Remove primers, dNTPs, polymerase and salts from PCR reactions in approximately 15 minutes with a manual silica spin-column clean-up workflow. The four-step bind, wash and elute process handles DNA fragments from 150 bp to 5 kb and concentrates them in a 40 µL elution. Purified DNA is ready for Sanger sequencing, cloning, enzymatic reactions and gel-free fragment analysis.
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IsoBind Plasmid Isolation Kit 50-Prep
Prepare clean plasmid DNA from 1.5 mL overnight bacterial cultures in approximately 30 minutes with a manual silica spin-column workflow. Alkaline lysis separates plasmid DNA from genomic DNA, RNA and protein before column binding and washing. The purified plasmid DNA is ready for restriction digestion, ligation, transformation, Sanger sequencing and routine cloning.
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IsoBind FFPE DNA Kit (IVD) 100-Prep
Recover amplifiable DNA from 5-10 µm FFPE tissue sections with a manual silica spin-column workflow that avoids xylene. Deparaffinisation and heat-based de-crosslinking address paraffin and formalin crosslinks before the DNA is captured and washed. The approximately 60-minute workflow prepares DNA for oncology mutation profiling, somatic variant analysis and targeted sequencing.
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IsoBind Marine Tissue RNA Kit 100-Prep
Recover total RNA from fish, shellfish and other marine tissues with a manual silica spin-column workflow formulated for lipid-rich and salt-rich samples. The lysis chemistry and increased reducing conditions help limit lipid oxidation and reduce salt and secondary-metabolite interference. The approximately 45-minute workflow prepares RNA for RT-qPCR, RNA-seq and aquatic transcriptomics.
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IsoBind Avocado RNA Kit 100-Prep
Recover analysis-ready total RNA from avocado leaf, flesh and peel with a manual silica spin-column workflow designed for lipid-rich and phenolic tissue. A Poly Trap step removes lipids, polyphenols and secondary metabolites before column binding, helping difficult avocado samples move cleanly into RT-qPCR and sequencing. The workflow takes approximately 45 minutes on a standard laboratory centrifuge.
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IsoBind VineVantage RNA Kit 100-Prep
Prepare total RNA from 80 to 100 mg frozen grapevine leaf or berry tissue with a manual silica spin-column workflow developed for difficult vine matrices. Clarification, volume-adjusted binding chemistry and three wash stages reduce compounds that can interfere with reverse transcription and amplification. The approximately 45-minute workflow prepares RNA for RT-qPCR, gene expression analysis and RNA sequencing.
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IsoBind RootRibose RNA Kit 100-Prep
Recover total RNA from potato, cassava and sweet potato roots with a manual silica spin-column workflow designed for starch-rich tissue. A Poly Trap step removes starch and polysaccharides before column binding, reducing the compounds that can suppress reverse transcription and amplification. The approximately 45-minute workflow prepares RNA for RT-qPCR, RNA-seq and root-crop pathogen detection.
