Next generation sequencing

Recover analysis-ready total RNA from avocado leaf, flesh and peel with a manual silica spin-column workflow designed for lipid-rich and phenolic tissue. A Poly Trap step removes lipids, polyphenols and secondary metabolites before column binding, helping difficult avocado samples move cleanly into RT-qPCR and sequencing. The workflow takes approximately 45 minutes on a standard laboratory centrifuge.