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IsoBind Marine Tissue RNA Kit 100-Prep

Recover total RNA from fish, shellfish and other marine tissues with a manual silica spin-column workflow formulated for lipid-rich and salt-rich samples. The lysis chemistry and increased reducing conditions help limit lipid oxidation and reduce salt and secondary-metabolite interference. The approximately 45-minute workflow prepares RNA for RT-qPCR, RNA-seq and aquatic transcriptomics.

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa

The IsoBind Marine Tissue RNA Kit is built for aquatic biology and molecular research laboratories that need a manual silica spin-column method for marine tissues with challenging lipid and salt content.

Homogenised tissue is lysed under reducing conditions before the cleared lysate is applied to the silica membrane. The chemistry is formulated to address lipid interference, salts and marine secondary metabolites, followed by wash steps and a 50-100 µL elution.

The kit processes fish, shellfish and other marine organism tissues, including high-lipid and salt-rich aquatic samples. The lyse, bind, wash and elute workflow takes approximately 45 minutes on a standard laboratory centrifuge.

Purified RNA is suitable for RT-qPCR, RNA-seq and transcriptomic studies. Local manufacture supports reliable supply for marine and aquaculture research programmes.

Sample type

Marine tissue

Nucleic acid

RNA

Format

Silica spin column

Workflow

Manual

Compatibility

Universal

Pack size

100 samples

Grade

Research (RUO)

Applications

Research, qPCR, RT-PCR, Next generation sequencing

Required, not supplied

Items you provide to complete the workflow.

  • Isopropanol
  • Absolute ethanol
  • Filter tips
  • Microtubes
  • Reducing agent (DTT, BME)
  • DNase
  • Pipettes
  • Microcentrifuge ≥10,000×g
  • Vortex
  • Heating block / thermoshaker
  • Sample crushing device
  • Steel beads for crushing
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