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IsoBind Viral RNA Kit (IVD) 100-Prep

Recover viral RNA from low-titre plasma, serum, urine, swab eluate and cell culture supernatant with a manual silica spin-column workflow. Booster-assisted binding and three wash steps help retain limited viral material while reducing haemoglobin, protein and chaotropic-salt carryover. The approximately 45-minute workflow prepares RNA for RT-qPCR, RT-PCR and next generation sequencing and is supplied for in vitro diagnostic use.

Specialists in the field of nucleic acid extractions

  • only manufacturer of such products in Africa
  • 1-3 Day Delivery times in South Africa

The IsoBind Viral RNA Kit is designed for diagnostic and research laboratories that need manual silica spin-column purification from low-input or low-titre viral specimens.

A 200 µL liquid sample is lysed with Proteinase K before Booster-assisted binding to the silica membrane. Three wash stages reduce haemoglobin, proteins and residual chaotropic salts, followed by a 50 µL elution.

The kit processes plasma, serum, urine, swab eluate and cell culture supernatant, with tissue as a secondary input after a suitable pre-lysis step. The workflow takes approximately 45 minutes for 1-24 samples on a standard laboratory centrifuge and is supplied for in vitro diagnostic use.

Purified RNA is suitable for RT-qPCR, RT-PCR and next generation sequencing. Manufactured to international standards, with reliable local supply.

Sample type

Viral

Nucleic acid

RNA

Format

Silica spin column

Workflow

Manual

Compatibility

Universal

Pack size

100 samples

Grade

Medical (IVD)

Applications

Diagnostics, PCR, qPCR, RT-PCR, Next generation sequencing, Healthcare

Required, not supplied

Items you provide to complete the workflow.

  • Isopropanol
  • Absolute ethanol
  • Filter tips
  • Microtubes
  • Reducing agent (DTT, BME)
  • DNase
  • Pipettes
  • Microcentrifuge ≥10,000×g
  • Vortex
  • Heating block / thermoshaker
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